Important stains introduction

1. Lipid or fat - Sudan black or oil red O

2. Calcium - von kossa or alzarine red s

3. Haemosiderin or iron - Prussian blue or Pearls stain

4. Collagen - Masson trichrome

5. Mucin - mucicarmine, alcian blue

6. Fungi - PAS, silver methanamine

7. Melanin - Masson Fontana

8. Mast cells - Toluidine blue

9. Amyloid - Congo red

10. Basement membrane - PAS

11. Nucleic acid or DNA - feulgen, acridine orange

12. Routine histo pathology - hematoxylin and eosin ( H&E)

13. Routine peripheral smear - geimsa or leishman

14. Lymphoblast - PAS

15. Myeloblasts - myeloperoxidase, non specific esterase, Sudan black B